THEOBROMINE

A xanthine alkaloid found predominantly in cacao that acts as a mild stimulant, vasodilator, and bronchodilator, providing smoother and longer-lasting effects compared to caffeine.

Theobromine is a methylxanthine alkaloid found naturally in cocoa, chocolate, and tea that acts as a mild stimulant and bronchodilator. It works by blocking adenosine receptors and inhibiting phosphodiesterase enzymes, producing gentler effects than caffeine with a longer half-life. Theobromine is primarily used for cardiovascular health, mood enhancement, and as a mild cognitive stimulant.

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Draw Volume
0.100mL
Syringe Units
10units
Concentration
2,500mcg/mL
Doses / Vial
20doses
Vial Total
5mg
Waste / Vial
0mcg
Syringe Cap.
100units · 1mL
How to reconstitute
Gather & prepare
1/6Gather & prepare

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1.Wash hands thoroughly, put on disposable gloves
2.Your 5mg peptide vial (lyophilized powder)
3.Bacteriostatic water (you'll need 2mL)
4.A 3–5mL syringe with 21–25 gauge needle for reconstitution
5.Alcohol swabs (70% isopropyl)
Use bacteriostatic water (0.9% benzyl alcohol) for multi-dose vials. Sterile water is only safe for single-use.
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Store 2-8°C30 day shelf lifeSwirl gentlyFor research purposes only

Research

Reported Effects

Cardiovascular Health:: Strong research evidence for improving lipid profiles and metabolic markers at 450mg daily doses. Cognitive Effects:: Inconsistent results across studies; failed to show reliable effects on vigilance and mood in controlled trials. Synergy with Caffeine:: May work better in combination with caffeine, particularly at higher caffeine intake levels. Dosage-Dependent:: Effects appear dose-dependent but optimal cognitive dosing remains unclear based on current evidence

  • Strong research evidence for improving lipid profiles and metabolic markers at 450mg daily doses
  • Inconsistent results across studies; failed to show reliable effects on vigilance and mood in controlled trials
  • May work better in combination with caffeine, particularly at higher caffeine intake levels
  • Effects appear dose-dependent but optimal cognitive dosing remains unclear based on current evidence

Safety Profile

Safety Profile: Theobromine

Common Side Effects

  • Mild diuretic effect and increased urination
  • Nausea and gastrointestinal discomfort, especially at doses above 500 mg/day
  • Headache and mild restlessness (milder than caffeine due to weaker CNS stimulation)
  • Increased heart rate at higher doses; theobromine is a weaker cardiac stimulant than caffeine
  • Mild heartburn or acid reflux, as theobromine relaxes the lower esophageal sphincter

Serious Adverse Effects

  • Cardiac arrhythmias at high doses (>1000 mg); theobromine has direct positive chronotropic and inotropic effects
  • Theobromine poisoning: tremors, sweating, severe nausea, vomiting, and headache at toxic doses (estimated threshold ~1000 mg in sensitive individuals)
  • Seizures reported in cases of extreme overdose
  • Unlike caffeine, theobromine has a longer half-life (6–10 hours), so effects may accumulate with frequent dosing
  • Possible exacerbation of GERD symptoms due to esophageal sphincter relaxation

Contraindications

  • Known hypersensitivity to theobromine or methylxanthines
  • Severe cardiac arrhythmias or unstable angina
  • Active gastroesophageal reflux disease (GERD) or esophageal conditions
  • Pregnancy (crosses the placenta; associated with lower birth weight in some epidemiological studies at high intakes)
  • Concurrent high-dose caffeine or theophylline use (methylxanthine toxicity risk)

Drug Interactions

  • Theophylline: Same metabolic pathway (CYP1A2); additive methylxanthine toxicity possible
  • Caffeine: Additive cardiovascular and CNS stimulatory effects
  • Adenosine: As a methylxanthine, theobromine may antagonize adenosine; may reduce efficacy of pharmaceutical adenosine
  • Beta-blockers: May partially counteract the cardiac effects of beta-blockers
  • CYP1A2 inhibitors (fluvoxamine, ciprofloxacin): May slow theobromine metabolism and increase plasma levels

Population-Specific Considerations

  • Elderly: Reduced methylxanthine clearance; use lower doses; monitor cardiac rhythm
  • Pediatric: Present in chocolate; generally safe at dietary levels but supplemental doses should be avoided
  • GERD patients: Avoid or minimize due to lower esophageal sphincter relaxation
  • Cardiac patients: Use with caution; monitor heart rate and rhythm
  • Pregnant women: Moderate dietary chocolate intake generally considered safe, but high supplemental doses should be avoided

Pharmacokinetic Profile

Molecular Structure

2D Structure
THEOBROMINE molecular structure
Molecular Properties
Formula
C7H8N4O2
Weight
180.16 Da
PubChem CID
5429
Exact Mass
180.0647 Da
LogP
-0.8
TPSA
67.2 Ų
H-Bond Donors
1
H-Bond Acceptors
3
Rotatable Bonds
0
Complexity
267
Identifiers (SMILES, InChI)
InChI
InChI=1S/C7H8N4O2/c1-10-3-8-5-4(10)6(12)9-7(13)11(5)2/h3H,1-2H3,(H,9,12,13)
InChIKeyYAPQBXQYLJRXSA-UHFFFAOYSA-N

Safety Profile

Common Side Effects

  • Sleep Disruption:: May interfere with sleep quality when taken later in the day due to long half-life
  • Minimal Acute Effects:: Generally well-tolerated with fewer side effects than caffeine
  • Individual Sensitivity:: Effects vary significantly between individuals
  • Toxicity in Pets:: Highly toxic to dogs and cats; safe storage required in households with pets

References (5)

  1. [1]
    The Therapeutic Potential of Theobromine in Obesity: A Comprehensive Review

    Review examining theobromine's potential therapeutic applications in obesity treatment, particularly its effects on metabolism and chronic low-grade inflammation associated with obesity.

  2. [2]
    Theobromine supplementation in combination with a low-calorie diet improves cardiovascular risk factors in overweight and obese subjects with metabolic syndrome: a randomized controlled trial

    12-week randomized trial showing 450mg daily theobromine supplementation significantly decreased waist circumference, improved lipid ratios (LDL-c/HDL-c, TG/HDL-c, TC/HDL-c), and increased HDL-c compared to placebo in metabolic syndrome patients.

  3. [3]
    A comprehensive insight into the molecular effect of theobromine on cardiovascular-related risk factors: A systematic review of in vitro and in vivo studies

    Systematic review examining molecular mechanisms of theobromine's beneficial effects on cardiovascular risk factors including lipid profile, glycemic status, and inflammatory markers.

  4. [5]
    Effects of Theobromine and Caffeine on Mood and Vigilance

    Double-blind study of 24 men testing theobromine doses (100, 200, 400mg) against caffeine found theobromine failed to consistently affect mood or vigilance parameters at any dose, while 100mg caffeine showed significant effects.

  5. [4]
    Association between Dietary Theobromine and Cognitive Function in a Representative American Population: A Cross-Sectional Study

    Analysis of 2,845 participants aged ≥60 years found the highest quantile of theobromine intake was positively associated with delayed recall, animal fluency, and digit symbol substitution test scores, suggesting beneficial effects on cognitive performance.

Updated 2026-03-08Sources: peptidebay, pubchem

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